Sulphydryl groups in proteins

The sulphydryl groups of sickle-cell haemoglobin.

particularpH owing to the screening ofthe haematin iron atom from the influence of the various ionizing groups. On this basis a larger critical I would signify that a larger concentration of salt ions is required for complete screening, and hence that there is a stronger interaction between the iron and the ionizing groups in MetHb. In conclusion, although the mechanism may be different it may ...

Reactivities of the sulphydryl groups of dog hemoglobin.

Dog hemoglobin has four sulphydryl groups at positions olfl (G18) and p93 (F9), all of which are titratable with mercurials. Only two of these, however, react with non-mercurial sulphydryl reagents. Kinetic results indicate that the reacting site might be the p”” (F9). An examination of the environment of the alI1 (G18) shows that this sulphydryl must be unreactive towards non-mercurials becaus...

Studies on induction by substances containing sulphydryl groups in post-nodal pieces of chick blastoderm.

The importance of sulphydryls in morphogenesis is being increasingly recognized. In the study of the role of thiols in amphibian and avian development one of the methods of approach has been to subject early embryonic stages to the action of reagents such as chloroacetophenone, monoiodoacetic acid, oxidized glutathione and mercaptoethanol (Beatty, 1949; Rapkine & Brachet, 1951; Deuchar, 1957; B...

Sulfhydryl Groups in Proteins

Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...

A study of the 'reactive' sulphydryl groups of adenosine 5'-triphosphate-creatine phosphotransferase.